Lancaster City will take on Prestwich Heys in the second round of the Lancashire FA Challenge Trophy.
The teams played each other in the final of the 2019/20 trophy, which was delayed due to Covid.
The game, eventually played in August 2021 at Leyland County Ground, ended 6-1 to the Dolly Blues.
It was the seventh time in their history that they had won the trophy, and the first since 1975.
Prestwich Heys play in the North West Counties League Premier Division - the ninth tier of English football - and are currently fifth, just four points off leaders Bury with a game in hand.
They finished seventh in the league last season.
Lancaster City did not play at the weekend - their trip to Marske United was postponed due to a waterlogged pitch at Mount Pleasant.
City's last game was a 2-1 home win over Ashton United last Tuesday (November 14), which was just their second win in eleven games.
It was a result that saw Mark Fell’s side climb to 11th in the NPL Premier Division, but they have since dropped to 13th, as teams around them played and picked up results on Saturday.
A fan favourite may make his on-the-pitch return to The Giant Axe on Tuesday after Lancaster announced his re-signing on Thursday (November 16).
Striker Tom Kilifin has returned to the club following his departure from Bury.
The forward has previously made 250 appearances across two spells at City, scoring 75 goals.
He played for another North West Counties League side in Kendal Town last season, netting 33 times in 44 games.
Lancaster City v Prestwich Heys in the Lancashire FA Challenge Trophy Second Round kicks off at 7.45pm this evening (November 21) at the Giant Axe.